Premium
Influence of nanoparticle‐embedded polymeric surfaces on cellular adhesion, proliferation, and differentiation
Author(s) -
Ventrelli Letizia,
Fujie Toshinori,
Turco Serena Del,
Basta Giuseppina,
Mazzolai Barbara,
Mattoli Virgilio
Publication year - 2014
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34935
Subject(s) - materials science , adhesion , cell adhesion , nanoparticle , wetting , nanotechnology , tissue engineering , regenerative medicine , magnetic nanoparticles , myogenesis , surface modification , myocyte , chemical engineering , biomedical engineering , cell , microbiology and biotechnology , composite material , chemistry , biochemistry , medicine , engineering , biology
The development of functional substrates to direct cellular organization is important for biomedical applications such as regenerative medicine and biorobotics. In this study, we prepared freestanding polymeric ultrathin films (nanofilms) consisting of poly(lactic acid) (PLA) and magnetic nanoparticles (MNPs), and evaluated the effects of their surface properties on the organization of cardiac‐like rat myoblasts (H9c2). We changed surface properties of the PLA nanofilms (i.e., roughness and wettability) as a function of MNPs concentration. We found that the incorporation of MNPs into the nanofilms enhanced both proliferation and adhesion of H9c2 cells. Through the morphological assessment of the differentiated H9c2 cells, we also found that the presence of MNPs significantly increased the fusion index and the surface area of myotubes. In conclusion, the embedding of MNPs is a simple method to tailor the physicochemical properties of the polymeric nanofilms, yet it is an effective approach to enhance the cellular morphogenesis in the field of cardiac tissue engineering for regenerative medicine and biorobotics applications. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2652–2661, 2014.