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Vascular endothelial growth factor‐loaded injectable hydrogel enhances plasticity in the injured spinal cord
Author(s) -
Rieux Anne,
Berdt Pauline,
Ansorena Eduardo,
Ucakar Bernard,
Damien Jacobs,
Schakman Olivier,
Audouard Emilie,
Bouzin Caroline,
Auhl Dietmar,
SimónYarza Teresa,
Feron Olivier,
BlancoPrieto Maria J.,
Carmeliet Peter,
Bailly Christian,
Clotman Fréderic,
Préat Véronique
Publication year - 2014
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34915
Subject(s) - self healing hydrogels , spinal cord , neurite , vascular endothelial growth factor , materials science , angiogenesis , regeneration (biology) , ex vivo , spinal cord injury , biomedical engineering , in vivo , medicine , chemistry , microbiology and biotechnology , in vitro , biology , cancer research , vegf receptors , neuroscience , polymer chemistry , biochemistry
We hypothesized that vascular endothelial growth factor (VEGF)‐containing hydrogels that gelify in situ after injection into a traumatized spinal cord, could stimulate spinal cord regeneration. Injectable hydrogels composed of 0.5% Pronova UPMVG MVG alginate, supplemented or not with fibrinogen, were used. The addition of fibrinogen to alginate had no effect on cell proliferation in vitro but supported neurite growth ex vivo . When injected into a rat spinal cord in a hemisection model, alginate supplemented with fibrinogen was well tolerated. The release of VEGF that was incorporated into the hydrogel was influenced by the VEGF formulation [encapsulated in microspheres or in nanoparticles or in solution (free)]. A combination of free VEGF and VEGF‐loaded nanoparticles was mixed with alginate:fibrinogen and injected into the lesion of the spinal cord. Four weeks post injection, angiogenesis and neurite growth were increased compared to hydrogel alone. The local delivery of VEGF by injectable alginate:fibrinogen‐based hydrogel induced some plasticity in the injured spinal cord involving fiber growth into the lesion site. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2345–2355, 2014.