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Formation of thermo‐sensitive polyelectrolyte complex micelles from two biocompatible graft copolymers for drug delivery
Author(s) -
Li Guiying,
Meng Yanfeng,
Guo Lei,
Zhang Ting,
Liu Junshen
Publication year - 2014
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34894
Subject(s) - micelle , lower critical solution temperature , materials science , copolymer , polyelectrolyte , drug delivery , chemical engineering , poly(n isopropylacrylamide) , carboxymethyl cellulose , polymer chemistry , drug carrier , critical micelle concentration , chitosan , nanotechnology , organic chemistry , polymer , chemistry , composite material , sodium , aqueous solution , metallurgy , engineering
Thermo‐sensitive polyelectrolyte complex (PEC) micelles assembled from two biocompatible graft copolymers chitosan‐ g ‐poly( N ‐isopropylacrylamide) (CS‐ g ‐PNIPAM) and carboxymethyl cellulose‐ g ‐poly( N ‐isopropylacrylamide) (CMC‐ g ‐PNIPAM) were prepared for delivery of 5‐fluorouracil (5‐FU). The PEC micelles showed a narrow size distribution with core‐shell structure, in which the core formed from positively charged CS and negatively charged CMC by electrostatic interactions and the shell formed from thermo‐sensitive PNIPAM. The synthesized PEC micelles have lower critical solution temperatures (LCST) in the region of 37°C, which is favorable for smart drug delivery applications. The hydrogen bondings between PEC micelles and 5‐FU increased the drug loading. Changing temperature, pH or ionic strength, a sustained and controlled release was observed due to the deformation of PEC micelles. Adding glutaraldehyde, a chemical crosslinking reagent, was an efficient way to reinforce the micelles structure and decrease the initial burst release. Cytotoxicity assays showed that drug‐loaded PEC micelles retained higher cell inhibition efficiency in HeLa cells. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2163–2172, 2014.