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Improved brain delivery of vincristine using dextran sulfate complex solid lipid nanoparticles: Optimization and in vivo evaluation
Author(s) -
Aboutaleb Ehsan,
Atyabi Fatemeh,
Khoshayand Mohammad Reza,
Vatanara Ali Reza,
Ostad Seyed Nasser,
Kobarfard Farzad,
Dinarvand Rassoul
Publication year - 2014
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34890
Subject(s) - solid lipid nanoparticle , zeta potential , materials science , in vivo , drug delivery , box–behnken design , chromatography , nanoparticle , pharmacology , chemistry , response surface methodology , nanotechnology , medicine , microbiology and biotechnology , biology
Vincristine (VC) sulfate, a freely water‐soluble cytotoxic agent was incorporated into cetyl palmitate solid lipid nanoparticles (SLNs) with the aid of dextran sodium sulfate (DS), a poly anion, using a microemulsion method. The manufacturing process was optimized using response surface methodology (Box–Behnken design). SLNs were characterized for size, zeta potential, morphology, crystallinity, and release behavior. The drug encapsulation efficiency reached up to 93% and release study revealed sustained drug release. SLN formulation showed comparable cytotoxic effect in comparison to VC sulfate solution against the MDA‐MB‐231 cells. The in vivo studies following injection to rat revealed higher plasma and tissue concentrations and longer drug mean residence times compared to VC solution. Using cumarin‐6 as a model drug, it was shown that drug delivery to the brain was enhanced close to five times using SLNs prepared in this study compared to free cumarin‐6. It can be concluded that complexes of cetyl palmitate SLNs with DS could produce high VC‐loaded SLNs suitable for delivery of anticancer drugs to brain tumors. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2125–2136, 2014.