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Tissue response to composite hydrogels for vertical bone augmentation in the rat
Author(s) -
Kinard Lucas A.,
Dahlin Rebecca L.,
Henslee Allan M.,
Spicer Patrick P.,
Chu ChiaYe,
Tabata Yasuhiko,
Beucken Jeroen J.J.P.,
Jansen John A.,
Young Simon,
Wong Mark E.,
Kasper F. Kurtis,
Mikos Antonios G.
Publication year - 2014
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34878
Subject(s) - materials science , biomedical engineering , self healing hydrogels , gelatin , resorption , ethylene glycol , parietal bone , composite number , animal model , bone formation , animal study , bone morphogenetic protein 2 , bone resorption , composite material , anatomy , surgery , skull , chemistry , medicine , polymer chemistry , in vitro , pathology , biochemistry , organic chemistry
The objective of the present study was to develop a preclinical animal model for evaluating bone augmentation and to examine the level of bone augmentation induced by hydrogel composites. Design criteria outlined for the development of the animal model included rigid immobilization of bilateral implants apposed to the parietal bone of the rat, while avoiding the calvarial sutures. The animal model was evaluated through the implantation of hydrogel composites of oligo(poly(ethylene glycol) fumarate) (OPF) and gelatin microparticles releasing bone morphogenetic protein‐2 (BMP‐2). The BMP‐2 release profile was varied and compared to the implantation of a material control without BMP‐2. Each hydrogel composite was implanted within a polypropylene cassette, which was immobilized to the calvarial bone using screws, and empty cassettes were implanted as a control. The design criteria for the animal model were realized; however, the level of bone augmentation did not vary between any of the groups after 4 weeks. Osteoclastic bone resorption occurred to a higher extent in groups releasing BMP‐2, but the cause could not be elucidated. In conclusion, a promising bone augmentation model was established in the rat; however, refinement of the hydrogel composites was suggested to optimize the constructs for bone augmentation applications. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2079–2088, 2014.