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Immunocompatibility evaluation of hydrogel‐coated polyimide implants for applications in regenerative medicine
Author(s) -
Sirova Milada,
Vlierberghe Sandra Van,
Matyasova Veronika,
Rossmann Pavel,
Schacht Etienne,
Dubruel Peter,
Rihova Blanka
Publication year - 2014
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34873
Subject(s) - materials science , polyimide , regenerative medicine , biomedical engineering , composite material , layer (electronics) , stem cell , medicine , biology , genetics
Immunocompatibility of gelatin‐based hydrogels to be applied as implant coatings for local regenerative treatment has been studied. First, the bio‐ and immuno‐acceptability of the methacrylamide‐modified gelatin hydrogels per se was screened. The results indicated that the hydrogels support cell growth. Metabolic activity of normal cells and permanent cell lines representing various cell types (endothelial, epithelial, fibroblast, and monocyte/macrophage) cultivated on the gelatin hydrogels was moderately lower compared to cells cultivated on tissue culture plastic. The cells cultivated on the hydrogels produced identical cytokines as the control cells although at lower levels. Importantly, no inflammatory activity, measured by nitric oxide and pro‐inflammatory cytokine (IL‐1α, IL‐6, and TNFα) production, was observed in peritoneal cells and monocyte/macrophage RAW 264.7 cell line cultivated on the hydrogels. Finally, polyimide (PI) implantable membranes were surface‐modified with gelatin hydrogels and screened for their in vivo immunocompatibility. Their histological examination performed after subcutaneous implantation in mice produced a sound proof of immunoacceptability. Normal tissue repair, mild cellular infiltration and edema mainly induced by the surgery were observed after 2 and 6 days. No adverse tissue responses were induced by the implants. Analysis performed after 4 and 9 weeks indicated areas of foreign body granuloma without formation of a fibrous capsule. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 1982–1990, 2014.