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Synthesis of SiO 2 system via sol–gel process: Biocompatibility tests with a fibroblast strain and release kinetics
Author(s) -
Catauro Michelina,
Bollino Flavia,
Papale Ferdinando
Publication year - 2014
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34836
Subject(s) - biocompatibility , materials science , kinetics , biomaterial , chemical engineering , simulated body fluid , amorphous solid , fibroblast , nuclear chemistry , biomedical engineering , composite material , scanning electron microscope , nanotechnology , chemistry , organic chemistry , biochemistry , in vitro , medicine , physics , quantum mechanics , engineering , metallurgy
Abstract SiO 2 glass has been synthesized via sol–gel process and enriched with 5 wt % sodium ampicillin. To verify the biocompatibility of the obtained biomaterial, fibroblasts have been grown on a glass surface and were tested for viability after 24 h. The results of the Water‐Soluble Tetrazolium (WST)‐8 analysis suggest that SiO 2 glass has an adequate biocompatibility. The amorphous nature of the gels has been ascertained by X‐ray diffraction analysis. Release kinetics have been subsequently investigated in a simulated body fluid. The amount of sodium ampicillin released has been detected by ultraviolet–visible spectroscopy. The release kinetics seems to occur in more than one stage. High‐performance liquid chromatography analysis has also been carried out to ensure the integrity of ampicillin after the synthesis treatment. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 1677–1680, 2014.