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Biocompatibility assessment of porous chitosan‐Nafion and chitosan‐PTFE composites in vivo
Author(s) -
Liu BoJi,
Ma LiNan,
Su Juan,
Jing WeiWei,
Wei MinJie,
Sha XianZheng
Publication year - 2014
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34830
Subject(s) - materials science , chitosan , polytetrafluoroethylene , biocompatibility , nafion , composite material , coating , biomedical engineering , porosity , tissue engineering , chemical engineering , chemistry , electrochemistry , medicine , electrode , engineering , metallurgy
Chitosan (CS) is widely used as a scaffold material in tissue engineering. The objective of this study was to test whether porous chitosan membrane (PCSM) coating for Nafion used in implantable sensor reduced fibrous capsule (FC) density and promoted superior vascularization compared with PCSM coating for polytetrafluoroethylene (PTFE). PCSM was fabricated with solvent casting/particulate leaching method using silica gel as porogen and characterized in vitro . Then, PCSM‐Nafion and PCSM‐PTFE composites were assembled with hydrated PCSM and implanted subcutaneously in rats. The histological analysis was performed in comparison with Nafion and PTFE. Implants were explanted 35, 65, and 100 days after the implantation. Histological assessments indicated that both composites achieved presumed effects of porous coatings on decreasing collagen deposition and promoting angiogenesis. PCSM‐PTFE exerted higher collagen deposition by area ratio, both within and outside, compared with that of PCSM‐Nafion. Angiogenesis within and outside the PCSM‐Nafion both increased over time, but that of the PCSM‐PTFE within decreased. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2055–2060, 2014.