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Plasma‐assisted heparin conjugation on electrospun poly( l ‐lactide) fibrous scaffolds
Author(s) -
Cheng Q.,
Komvopoulos K.,
Li S.
Publication year - 2014
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34802
Subject(s) - surface modification , materials science , derivatization , heparin , lactide , electrospinning , covalent bond , polymer chemistry , chemical engineering , polymerization , nuclear chemistry , polymer , chemistry , organic chemistry , biochemistry , composite material , high performance liquid chromatography , engineering
Heparin conjugation on poly( l ‐lactide) fibrous scaffolds fabricated by electrospinning was accomplished by surface functionalization with amine (–NH 2 ) groups using a sequential treatment with Ar‐NH 3 and H 2 plasmas. The density of the incorporated –NH 2 groups was determined by combining a chemical derivatization method with X‐ray photoelectron spectroscopy. The time of Ar‐NH 3 plasma treatment significantly affected the N/C, –NH 2 /N, and –NH 2 /C fractions, whereas the plasma power, Ar‐NH 3 gas composition, and time of H 2 plasma treatment only influenced the –NH 2 /N and –NH 2 /C fractions. Scaffold surface functionalization by –NH 2 groups significantly increased the amount of covalently bonded heparin compared to a hydrolysis method. The function of immobilized heparin was confirmed by the decrease of platelet attachment during the exposure of the scaffolds to blood from Sprague‐Dawley rats. In vitro experiments with bovine aorta endothelial cells demonstrated that heparin conjugation enhanced cell infiltration through the fibrous scaffolds, regardless of the amount of covalently immobilized heparin. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 1408–1414, 2014.