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In vitro response to alkaline phosphatase coatings immobilized onto titanium implants using electrospray deposition or polydopamine‐assisted deposition
Author(s) -
Nijhuis Arnold W. G.,
van den Beucken Jeroen J.J.P.,
Jansen John A.,
Leeuwenburgh Sander C. G.
Publication year - 2014
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34776
Subject(s) - alkaline phosphatase , materials science , titanium , mineralization (soil science) , osteoblast , osseointegration , deposition (geology) , simulated body fluid , biomolecule , electrospray , nanotechnology , chemical engineering , implant , biomedical engineering , in vitro , enzyme , chemistry , biochemistry , scanning electron microscope , metallurgy , composite material , chromatography , mass spectrometry , organic chemistry , biology , surgery , engineering , paleontology , medicine , sediment , nitrogen
Immobilization of biomolecules onto implant surfaces is one of the most straightforward strategies to control the interaction between an implant and its biological environment. Recently, it was shown that the enzyme alkaline phosphatase (ALP) could be efficiently immobilized onto titanium implants in a single step using polydopamine. We hypothesized that such polydopamine‐ALP coatings can enhance the early attachment of cells and increase mineralization. Therefore, the current study aimed at immobilization of ALP onto titanium by means of either one‐ or two‐step polydopamine‐assisted immobilization or electrospray deposition, the comparative characterization of these experimental substrates and subsequent cell behavioral analysis using primary osteoblast‐like cells. Uncoated titanium and ALP‐free polydopamine coatings served as controls. Despite significant ALP surface activity and lower water contact for angles ALP‐containing surface modifications, only marginal effects on early cell behavior (i.e., cell spreading) and osteogenic differentiation (i.e., proliferation, differentiation and mineralization) were observed in comparison to uncoated titanium. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 1102–1109, 2014.