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Synergistic effect between bioactive glass foam and a perfusion bioreactor on osteogenic differentiation of human adipose stem cells
Author(s) -
Silva A. R. P.,
Paula A. C. C.,
Martins T. M. M.,
Goes A. M.,
Pereria M. M.
Publication year - 2014
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34758
Subject(s) - osteopontin , osteocalcin , bioactive glass , alkaline phosphatase , materials science , tissue engineering , scaffold , microbiology and biotechnology , stem cell , biomedical engineering , adipose tissue , cell culture , bioreactor , cellular differentiation , osteoblast , in vitro , biophysics , chemistry , biochemistry , biology , immunology , medicine , botany , composite material , enzyme , genetics , gene
Tissue engineering is a multidisciplinary science that combines a structural scaffold and cells to form a construct able to promote regeneration of injured tissue. Bioactive glass foam produced by sol–gel is an osteoinductive material with a network of interconnected macropores necessary for cell colonization. The use of human adipose‐derived stem cell (hASC) presents advantages as the potential for a large number of cells, rapid expansion in vitro and the capability of differentiating into osteoblasts. The use of a bioreactor in three‐dimensional cell culture enables greater efficiency for cell nutrition and application of mechanical forces, important modulators of bone physiology. The hASC seeded in a bioactive glass scaffold and cultured in osteogenic Leibovitz L‐15 medium in a bioreactor with a flow rate of 0.1 mL min −1 demonstrated a significant increase in cell proliferation and viability and alkaline phosphatase (ALP) activity peak after 14 days. The immunofluorescence assay revealed an expression of osteopontin, osteocalcin and type I collagen from 7 to 21 days after culture. The cells changed from a spindle shape to a cuboidal morphology characteristic of osteoblasts. The polymerase chain reaction assay confirmed that osteopontin, osteocalcin, and ALP genes were expressed. These results indicate that hASCs differentiated into an osteogenic phenotype when cultured in bioactive glass scaffold, osteogenic Leibovitz L‐15 medium and a perfusion bioreactor. Therefore, these results highlight the synergism between a bioactive glass scaffold and the effect of perfusion on cells and indicate the differentiation into an osteogenic phenotype. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 818–827, 2014.

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