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Biofilm formation on nanostructured hydroxyapatite‐coated titanium
Author(s) -
Westas Emma,
Gillstedt Martin,
LönnStensrud Jessica,
Bruzell Ellen,
Andersson Martin
Publication year - 2014
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34757
Subject(s) - biofilm , materials science , titanium , staphylococcus epidermidis , adhesion , confocal laser scanning microscopy , coating , scanning electron microscope , nanomaterials , chemical engineering , nanotechnology , biomedical engineering , composite material , bacteria , metallurgy , medicine , genetics , engineering , biology , staphylococcus aureus
Biofilm formation on medical devices is a common cause of implant failure, especially regarding implants that breach the epithelial tissue, so‐called transcutaneous implants. Nanotechnology and the development of new nanomaterials have given the opportunity to design nanotextured implant surfaces. Such surfaces have been studied using various in vitro methods showing that nanosized features strongly benefit bone cell growth. However, little is known on how nanostructured features affect biofilm formation. The aim of this study was therefore to examine the shape‐ and chemical‐dependent effect of a nanostructured hydroxyapatite (HA) coating on the degree of Staphylococcus epidermidis biofilm formation. Three different types of nanosized HA particles having different shapes and calcium to phosphate ratios were compared to uncoated turned titanium using safranin stain in a biofilm assay and confocal laser scanning microscopy (CLSM) for assessment of biofilm biomass and bacterial volume, respectively. No difference in biofilm biomass was detected for the various surfaces after 6 h incubation with S. epidermidis . Additionally, image analysis of CLSM Z‐stacks confirmed the biofilm assay and showed similar results. In conclusion, the difference in nanomorphology and chemical composition of the surface coatings did not influence the adhesion and biofilm formation of S. epidermidis . © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 1063–1070, 2014.