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A new TGF‐β3 controlled‐released chitosan scaffold for tissue engineering synovial sheath
Author(s) -
Jiang Ke,
Wang Ziming,
Du Quanyin,
Yu Jiang,
Wang Aimin,
Xiong Yan
Publication year - 2014
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34742
Subject(s) - scaffold , materials science , biomedical engineering , adhesion , tissue engineering , tendon , chitosan , transforming growth factor , microbiology and biotechnology , anatomy , medicine , chemistry , biology , composite material , biochemistry
The post‐operative outcome of flexor tendon healing remains limited by flexor tendon adhesion that reduces joint range of motion. Despite improvement in different methods, peritendinous adhesion formation continues to present a formidable challenge. Recent studies showed that transforming growth factor‐β3 (TGF‐β3) may be the key factor to reducing adhesion formation in skin or tendon. In this study, we designed a novel type of tissue engineering synovial sheath containing TGF‐β3, to prevent flexor tendon adhesion. First, to achieve a stable release of TGF‐β3, chitosan microspheres, prepared by crosslinking‐emulsion, were used for the delivery of TGF‐β3. Second, a three‐dimensional chitosan scaffold was prepared by lyophilization, and TGF‐β3 microspheres were carefully introduced into the scaffold. Then, synovial cells were cultured and then seeded into the TGF‐β3 loaded scaffold to produce TGF‐β3 controlled‐released tissue engineering synovial sheath. Tests clearly demonstrated that the scaffold has good structure and compatibility with cells. These results expand the feasibility of combinative strategies of controlled protein release and tissue‐engineered synovial sheath formation. Application of this scaffold to tendon repair sites may help to prevent adhesion of tendon healing. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 801–807, 2014.