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Influence of cell culture medium composition on in vitro dissolution behavior of a fluoride‐containing bioactive glass
Author(s) -
Shah Furqan A.,
Brauer Delia S.,
Wilson Rory M.,
Hill Robert G.,
Hing Karin A.
Publication year - 2014
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34724
Subject(s) - dissolution , apatite , hepes , fluoride , nuclear chemistry , bioactive glass , in vitro , materials science , chemistry , inorganic chemistry , mineralogy , biochemistry , organic chemistry , composite material
Bioactive glasses are used clinically for bone regeneration, and their bioactivity and cell compatibility are often characterized in vitro , using physiologically relevant test solutions. The aim of this study was to show the influence of varying medium characteristics (pH, composition, presence of proteins) on glass dissolution and apatite formation. The dissolution behavior of a fluoride‐containing bioactive glass (BG) was investigated over a period of one week in Eagle's Minimal Essential Medium with Earle's Salts (MEM), supplemented with either, (a) acetate buffer, (b) 4‐(2‐hydroxyethyl)‐1‐piperazineethanesulfonic acid (HEPES) buffer, (c) HEPES + carbonate, or (d) HEPES + carbonate + fetal bovine serum. Results show pronounced differences in pH, ion release, and apatite formation over 1 week: Despite its acidic pH (pH 5.8 after BG immersion, as compared to pH 7.4–8.3 for HEPES‐containing media), apatite formation was fastest in acetate buffered (HEPES‐free) MEM. Presence of carbonate resulted in formation of calcite (calcium carbonate). Presence of serum proteins, on the other hand, delayed apatite formation significantly. These results confirm that the composition and properties of a tissue culture medium are important factors during in vitro experiments and need to be taken into consideration when interpreting results from dissolution or cell culture studies. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 647–654, 2014.