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Effects of water‐holding capability of the PVF sponge on the adhesion and differentiation of rat bone marrow stem cell culture
Author(s) -
Togami Wakana,
Sei Akira,
Okada Tatsuya,
Taniwaki Takuya,
Fujimoto Toru,
Nakamura Takayuki,
Tahata Shogo,
Nakanishi Yoshitaka,
Mizuta Hiroshi
Publication year - 2014
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34695
Subject(s) - sponge , materials science , adhesion , cell adhesion , stem cell , bone marrow , cell culture , biomedical engineering , microbiology and biotechnology , composite material , immunology , biology , botany , engineering , genetics
The aim of the study is to estimate the effects of the water‐holding capability of the polyvinyl formal (PVF) sponges on osteogenic response in vitro experiments. The rat bone marrow stem cells (BMCs) were seeded and cultured for up to 4 weeks under static conditions in osteogenic media to evaluate the adhesion, proliferation, differentiation, and mineralization on the Dextran‐coated PVF sponges with or without water‐holding capability. The BMCs seeded onto the PVF sponges with water‐holding capability showed more significant increases in DNA content, alkaline phosphatase (ALP) activity, osteocalcin content, and calcium deposition than those without water‐holding capability. These results suggest that the Dextran‐coated PVF sponges with high water‐holding capability would have potential uses as both a new scaffold to bone tissue engineering and as a new biomaterial. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 247–253, 2014.