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Characterization of chondrocyte scaffold carriers for cell‐based gene therapy in articular cartilage repair
Author(s) -
Shui Wei,
Yin Liangjun,
Luo Jeffrey,
Li Ruidong,
Zhang Wenwen,
Zhang Jiye,
Huang Wei,
Hu Ning,
Liang Xi,
Deng ZhongLiang,
Hu Zhenming,
Shi Lewis L.,
Luu Hue H.,
Haydon Rex C.,
He TongChuan,
Ho Sherwin H.
Publication year - 2013
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34661
Subject(s) - chondrocyte , cartilage , microbiology and biotechnology , materials science , viability assay , articular cartilage repair , matrix (chemical analysis) , luciferase , biomedical engineering , biology , cell culture , pathology , transfection , cell , anatomy , osteoarthritis , articular cartilage , medicine , biochemistry , alternative medicine , genetics , composite material
Articular cartilage lesions in the knee are common injuries. Chondrocyte transplant represents a promising therapeutic modality for articular cartilage injuries. Here, we characterize the viability and transgene expression of articular chondrocytes cultured in three‐dimensional scaffolds provided by four types of carriers. Articular chondrocytes are isolated from rabbit knees and cultured in four types of scaffolds: type I collagen sponge, fibrin glue, hyaluronan, and open‐cell polylactic acid (OPLA). The cultured cells are transduced with adenovirus expressing green fluorescence protein (AdGFP) and luciferase (AdGL3‐Luc). The viability and gene expression in the chondrocytes are determined with fluorescence microscopy and luciferase assay. Cartilage matrix production is assessed by Alcian blue staining. Rabbit articular chondrocytes are effectively infected by AdGFP and exhibited sustained GFP expression. All tested scaffolds support the survival and gene expression of the infected chondrocytes. However, the highest transgene expression is observed in the OPLA carrier. At 4 weeks, Alcian blue‐positive matrix materials are readily detected in OPLA cultures. Thus, our results indicate that, while all tested carriers can support the survival of chondrocytes, OPLA supports the highest transgene expression and is the most conductive scaffold for matrix production, suggesting that OPLA may be a suitable scaffold for cell‐based gene therapy of articular cartilage repairs. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 3542–3550, 2013.