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Biological characterization of oxidized hyaluronic acid/resveratrol hydrogel for cartilage tissue engineering
Author(s) -
Sheu ShiowYunn,
Chen WenShan,
Sun JuiSheng,
Lin FengHuei,
Wu Tuoh
Publication year - 2013
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34653
Subject(s) - biocompatibility , hyaluronic acid , chondrocyte , materials science , cartilage , extracellular matrix , aggrecan , tissue engineering , self healing hydrogels , biomedical engineering , viability assay , resveratrol , osteoarthritis , cell , chemistry , biochemistry , articular cartilage , polymer chemistry , biology , anatomy , medicine , pathology , alternative medicine , metallurgy
Osteoarthritis is a type of arthritis that is caused by breakdown of cartilage, with eventual loss of the cartilage of the joints. The ability of self‐repair in damaged cartilage tissue is limited; the aim of this work is to fabricate and characterize an oxidized hyaluronic acid/resveratrol (Oxi‐HA/Res) hydrogel for future applications in cartilage tissue engineering. Under physiological conditions, the Oxi‐HA/Res hydrogel was prepared by chemical crosslinking of Oxi‐HA with resveratrol solution and characterized by Fourier transform infrared spectrometry assay; the biocompatibility and gene expression of chondrocytes within the Oxi‐HA‐Res hydrogel then analyzed. The cell viability and cytotoxicity assays showed that the Oxi‐HA/Res hydrogel has good biocompatibility. Oxi‐HA/Res hydrogel can upregulate expression of type II collagen, aggrecan, and Sox‐9 genes; while down‐regulating IL‐1β, MMP‐1, MMP‐3, MMP13 gene expression. It can also reduce LPS‐induced inflammation and chondrocyte damage. The results of this study showed that the Oxi‐HA/Res hydrogel is biocompatible with chondrocytes, allows for extracellular matrix synthesis, and also reduce LPS‐induced inflammation and damage. These results suggest that Oxi‐HA/Res hydrogel may be a potential suitable cell carrier for chondrocyte cells in the treatment of cartilage defect. However, further in vivo study is mandatory for future possible clinical applications. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 3457–3466, 2013.