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The effect of porosity of a biphasic ceramic scaffold on human skeletal stem cell growth and differentiation in vivo
Author(s) -
Aarvold Alexander,
Smith James O.,
Tayton Edward R.,
Lanham Stuart A.,
Chaudhuri Julian B.,
Turner Irene G.,
Oreffo Richard O. C.
Publication year - 2013
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34646
Subject(s) - scaffold , porosity , materials science , in vivo , biomedical engineering , tissue engineering , stem cell , cell growth , biophysics , microbiology and biotechnology , chemistry , composite material , biology , biochemistry , medicine
Skeletal stem cell (SSC) growth on a novel porous HA/TCP scaffold has been investigated in vivo . The effect of porosity on osteogenic differentiation was assessed by comparing two groups of scaffolds with differing porosity but controlled pore size. Histology, microCT, scanning electron microscopy, and biochemical analysis were used to assess SSC proliferation and differentiation. The 45 pores per inch (ppi) scaffold demonstrated a greater increase in density than the 30 ppi scaffold following in vivo culture, and a reduction in dimensions of the pores and channels of the higher porosity scaffold was observed, indicating generation of new tissue within the pores. All scaffolds supported SSC proliferation but the higher scaffold porosity augmented osteogenic differentiation. ALP specific activity was enhanced on the 45 ppi scaffold compared to the 30 ppi scaffold. These studies demonstrate the importance of porosity in scaffold design and impact therein for tissue engineering application. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 3431–3437, 2013.