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Biphasic release of protein from polyethylene glycol and polyethylene glycol/modified dextran microspheres
Author(s) -
Nguyen Hoai X.,
O'Rear Edgar A.
Publication year - 2013
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34569
Subject(s) - polyethylene glycol , dextran , peg ratio , materials science , bovine serum albumin , microsphere , acetic anhydride , particle size , polysaccharide , albumin , polymer chemistry , chromatography , nuclear chemistry , chemistry , biochemistry , chemical engineering , catalysis , finance , engineering , economics
Dextrans show great promise for delivery of therapeutic agents. Dextran acetates (DAs) were synthesized with increasing degrees of substitution (DA1 < DA2 < DA3) by the reaction of the polysaccharide dextran (70 kDa) with acetic anhydride. A series of polyethylene glycol (PEG)/DA microspheres were prepared and tested with bovine serum albumin (BSA) functioning as a model protein. Particle size (0.74–0.85 μm) and encapsulation efficiency (56–70%) increased with the degree of substitution along with a slower release rate of protein from PEG/DA microspheres. Time to release 90% of protein rose from 31 to 118 min. Percentage of BSA released from PEG and PEG/DA3 microspheres with time (min) was modeled mathematically [ Y PEG = 100(1 − e −0.12 t ); Y PEG/DA3 = 100(1 − e −0.024 t )] to predict cumulative delivery from mixtures in vitro over a period of hours when constrained to a target level at 30 min. The system is examined for potential application in thrombolytic therapy. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 2699–2705, 2013.