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Osteoblastic cellular responses on ionically crosslinked chitosan‐tripolyphosphate fibrous 3‐D mesh scaffolds
Author(s) -
Pati Falguni,
Kalita Hemjyoti,
Adhikari Basudam,
Dhara Santanu
Publication year - 2013
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34559
Subject(s) - materials science , chitosan , composite material , biomedical engineering , biophysics , chemical engineering , medicine , engineering , biology
Tripolyphosphate (TPP) crosslinked chitosan (CH)‐based fibrous matrices have potential as bioactive scaffolds for bone tissue engineering. This study describes mechanical, biomineralization, and in vitro bone cell growth and differentiation properties of CH‐TPP (chitosan‐tripolyphosphate) fibrous scaffolds and compared with that of uncrosslinked CH one. The hydrated CH‐TPP scaffolds were viscoelastic in nature and their compressive strength was ∼2.9 MPa, which is greater than recent polymer experimental bone scaffolds. This improvement in mechanical properties of CH‐TPP scaffold may be beneficial toward cancellous bone graft application. Furthermore, CH‐TPP fibers supported in vitro biomineralization with phosphate as nucleation site; however, no significant difference in biomineralization morphology was observed with uncrosslinked CH fibers. Interestingly, a significant improvement in cellular responses (>33% increase in cell number based on DNA quantification) was observed when osteoblast like cells were cultured on the CH‐TPP scaffolds than that of CH scaffolds without phosphate group. Enhanced osteoblastic differentiation of MG63 cells on CH‐TPP scaffolds was also evidenced. Altogether, the results show that the CH‐TPP fibrous scaffolds are encouraging for bone tissue engineering. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 2526–2537, 2013.