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Phenotypic redifferentiation and cell cluster formation of cultured human articular chondrocytes in a three‐dimensional oriented gelatin scaffold in the presence of PGE 2 ‐ first results of a pilot study
Author(s) -
Brochhausen Christoph,
Sánchez Natalia,
Halstenberg Sven,
Zehbe Rolf,
Watzer Bernhard,
Schmitt Volker H.,
Hofmann Alexander,
Meurer Andrea,
Unger Ron E.,
Kirkpatrick Charles James
Publication year - 2013
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34538
Subject(s) - scaffold , cartilage , tissue engineering , gelatin , microbiology and biotechnology , regeneration (biology) , materials science , articular cartilage , prostaglandin e2 , cell growth , biomedical engineering , prostaglandin e , regenerative medicine , chondrocyte , phenotype , cell , chemistry , anatomy , osteoarthritis , stem cell , biology , pathology , biochemistry , medicine , endocrinology , alternative medicine , gene
Modern tissue engineering strategies comprise three elemental parameters: cells, scaffolds and growth factors. Articular cartilage represents a highly specialized tissue which allows frictionless gliding of corresponding articulating surfaces. As the regenerative potential of cartilage is low, tissue engineering‐based strategies for cartilage regeneration represent a huge challenge. Prostaglandins function as regulators in cartilage development and metabolism, especially in growth plate chondrocytes. In this study, it was analyzed if prostaglandin E 2 (PGE 2 ) has an effect on the phenotypic differentiation of human chondrocytes cultured in a three‐dimensional (3D) gelatin‐based scaffold made by directional freezing and subsequent freeze‐drying. As a result, it was clearly demonstrated that low doses of PGE 2 revealed beneficial effects on the phenotypic differentiation and collagen II expression of human articular chondrocytes in this 3D cell culture system. In conclusion, PGE 2 is an interesting candidate for tissue engineering applications since it represents an already well‐studied molecule which is available in pharmaceutical quality. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.