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Fabrication and characterization of regenerated silk scaffolds reinforced with natural silk fibers for bone tissue engineering
Author(s) -
Mobini Sahba,
Hoyer Birgit,
SolatiHashjin Mehran,
Lode Anja,
Nosoudi Nasim,
Samadikuchaksaraei Ali,
Gelinsky Michael
Publication year - 2013
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34537
Subject(s) - fibroin , materials science , silk , scaffold , composite material , tissue engineering , compressive strength , bombyx mori , composite number , fiber , biomedical engineering , chemistry , medicine , biochemistry , gene
Abstract We introduce a novel Bombyx mori silk‐based composite material developed for bone tissue engineering. Three‐dimensional scaffolds were fabricated by embedding of natural degummed silk fibers in a matrix of regenerated fibroin, followed by freeze‐drying. Different ratios of fibers to fibroin were investigated with respect to their influence on mechanical and biological properties. For all scaffold types, an interconnected porous structure suitable for cell penetration was proven by scanning electron microscopy. Compressive tests, carried out in static and cyclic mode under dry as well as wet conditions, revealed a strong impact of fiber reinforcement on compressive modulus and compressive stress. Cell culture experiments with human mesenchymal stem cells demonstrated that the fiber/fibroin composite scaffolds support cell attachment, proliferation, as well as differentiation along the osteoblastic lineage. Considering the excellent mechanical and biological properties, novel fiber/fibroin scaffolds appear to be an interesting structure for prospect studies in bone tissue engineering. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2013.