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Development of bioactive porous α‐TCP/HAp beads for bone tissue engineering
Author(s) -
Asaoka Teruo,
Ohtake Shoji,
Furukawa Katsuko S.,
Tamura Akito,
Ushida Takashi
Publication year - 2013
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34517
Subject(s) - materials science , scaffold , tissue engineering , bead , biomedical engineering , bone tissue , porosity , adhesion , composite material , cell adhesion , medicine
Porous beads of bioactive ceramics such as hydroxyapatite (HAp) and tribasic calcium phosphate (TCP) are considered a promising scaffold for cultivating bone cells. To realize this, α‐TCP/HAp functionally graded porous beads are fabricated with two main purposes: to maintain the function of the scaffold with sufficient strength up to the growth of new bone, and is absorbed completely after the growth. HAp is a bioactive material that has both high strength and strong tissue‐adhesive properties, but is not readily absorbed by the human body. On the contrary, α‐TCP is highly bioabsorbable, resulting in a scaffold that is absorbed before it is completely replaced by bone. In this study, we produced porous, bead‐shaped carriers as scaffolds for osteoblast culture. To control the solubility in vivo , the fabricated beads contained α‐TCP at the center and HAp at the surface. Cell adaptability of these beads for bone tissue engineering was confirmed in vitro . It was found that α‐TCP/HAp bead carriers exhibit low toxicity in the initial stages of cell seeding and cell adhesion. The presence of HAp in the composite bead form effectively increased ALP activity. In conclusion, it is suggested that these newly developed α‐TCP/HAp beads are a promising tool for bone tissue engineering. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 3295–3300, 2013.