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Magnesium incorporation into β‐TCP reduced its in vivo resorption by decreasing parathormone production
Author(s) -
Yassuda Debora H.,
Costa Neusa F. M.,
Fernandes Gustavo O.,
Alves Gutemberg G.,
Granjeiro José M.,
Soares Glória de A.
Publication year - 2013
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34502
Subject(s) - osteopontin , osteoprotegerin , osteocalcin , resorption , bone resorption , medicine , materials science , endocrinology , in vivo , activator (genetics) , receptor , chemistry , biology , biochemistry , alkaline phosphatase , microbiology and biotechnology , enzyme
Beta‐tricalcium phosphate (β‐TCP), one of the most widely used bioresorbable materials for bone therapy, can be doped with magnesium ions, generating β‐TCMP. The objectives of this work were to evaluate, on a murine dental alveolus grafting model, the biocompatibility of β‐TCP and β‐TMCP granules by histomorphometric analysis, as well as the impact on plasmatic levels of receptor activator of nuclear factor κB ligand (RANK‐L), osteoprotegerin (OPG), osteocalcin, osteopontin, and parathormone (PTH) during bone repair, using Luminex multiplexing technology. After grafting for 42 days, β‐TCP grafted group presented higher bioresorption and induced more newly formed bone than β‐TCMP ( p < 0.05). β‐TCP grafting also induced higher plasmatic levels of RANK‐L, compared to β‐TCMP and control (blood clot) groups at 21st day ( p < 0.05). PTH, which remained at low levels in control group, presented a time‐dependent increase in grafted groups, attaining significantly higher levels with β‐TCP by the 42nd day ( p < 0.05). RANK‐L/OPG ratio increased on β‐TCP group and attained a peak on the 21st day. In conclusion, β‐TCP granules were more bioresorbable and osteogenic than β‐TCMP granules, and the resorption of both materials might have been affected by osteoclastogenesis modulated by changes in the plasmatic levels of PTH and RANK‐L. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.