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Efficacy of mesoporous silica nanoparticles in delivering BMP‐2 plasmid DNA for in vitro osteogenic stimulation of mesenchymal stem cells
Author(s) -
Kim TaeHyun,
Kim Meeju,
Eltohamy Mohamed,
Yun YeRang,
Jang JunHyeog,
Kim HaeWon
Publication year - 2013
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34466
Subject(s) - bone morphogenetic protein 2 , mesenchymal stem cell , transfection , bone sialoprotein , osteopontin , materials science , osteocalcin , mesoporous silica , microbiology and biotechnology , bone morphogenetic protein 6 , bone morphogenetic protein 7 , in vitro , biophysics , alkaline phosphatase , bone morphogenetic protein , chemistry , mesoporous material , biology , biochemistry , gene , immunology , enzyme , catalysis
We report the ability of aminated mesoporous silica nanoparticles (MSN–NH 2 ) with large mesopore space and positive‐charged surface to deliver genes within rat mesenchymal stem cells (MSCs). The amine functionalized inorganic nanoparticles were complexed with bone morphogenetic protein‐2 (BMP2) plasmid DNA (pDNA) to study their transfection efficiency in MSCs. Intracellular uptake of the complex BMP2 pDNA/MSN–NH 2 occurred significantly, with a transfection efficiency of approximately 68%. Furthermore, over 66% of the transfected cells produced BMP2 protein. The osteogenic differentiation of the transfected MSCs was demonstrated by the expression of bone‐related genes and proteins including bone sialoprotein, osteopontin, and osteocalcin. The MSN–NH 2 delivery vehicle for BMP2 pDNA developed in this study may be a potential gene delivery system for bone tissue regeneration. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.