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In vitro cytotoxicity and in vivo osseointergration properties of compression‐molded HDPE‐HA‐Al 2 O 3 hybrid biocomposites
Author(s) -
Tripathi Garima,
Gough Julie E.,
Dinda Amit,
Basu Bikramjit
Publication year - 2013
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34452
Subject(s) - materials science , biocompatibility , high density polyethylene , in vivo , osteoblast , biomedical engineering , cytotoxicity , composite number , composite material , implant , in vitro , polyethylene , medicine , chemistry , surgery , biology , metallurgy , biochemistry , microbiology and biotechnology
The aim of this study was to investigate the in vivo biocompatibility in terms of healing of long segmental bone defect in rabbit model as well as in vitro cytotoxicity of eluates of compression‐molded High density polyethylene (HDPE)–hydroxyapatite (HA)‐aluminum oxide (Al 2 O 3 ) composite‐based implant material. Based on the physical property in terms of modulus and strength properties, as reported in our recent publication, HDPE‐40 wt % HA and HDPE‐20 wt % HA‐20 wt % Al 2 O 3 hybrid composites were used for biocompatibility assessment. Osteoblasts cells were cultured in conditioned media, which contains varying amount of composite eluate (0.01, 0.1, and 1.0 wt %). In vitro , the eluates did not exhibit any significant negative impact on proliferation, mineralization or on morphology of human osteoblast cells. In vivo , the histological assessment revealed neobone formation at the bone/implant interface, characterized by the presence of osteoid and osteoblasts. The observation of osteoclastic activity indicates the process of bone remodeling. No inflammation to any noticeable extent was observed at the implantation site. Overall, the combination of in vitro and in vivo results are suggestive of potential biomedical application of compression‐molded HDPE‐ 20 wt % HA‐ 20 wt % Al 2 O 3 composites to heal long segmental bone defects without causing any toxicity of bone cells. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.