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Sustained, localized transgene expression mediated from lentivirus‐loaded biodegradable polyester elastomers
Author(s) -
Jen Michele C.,
Baler Kevin,
Hood Ashleigh R.,
Shin Seungjin,
Shea Lonnie D.,
Ameer Guillermo A.
Publication year - 2013
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34449
Subject(s) - lentivirus , transgene , materials science , gene delivery , in vivo , regenerative medicine , tissue engineering , polyester , biocompatible material , biomaterial , biomedical engineering , microbiology and biotechnology , genetic enhancement , nanotechnology , chemistry , biology , biochemistry , stem cell , human immunodeficiency virus (hiv) , gene , immunology , medicine , composite material , viral disease
The study of biomaterials for gene delivery in tissue engineering and regenerative medicine is a growing area, necessitating the investigation of new biomaterials and gene delivery vectors. Poly(1,8‐octanediol citrate) (POC) and poly(glycerol‐sebacate) (PGS) are biodegradable, biocompatible elastomers that have tunable mechanical properties, surface characteristics, and degradation rate. The objective of this work was to investigate whether POC and PGS would support the immobilization and release of lentivirus to allow sustained and localized transgene expression. Porous biomaterials were prepared using salt as a porogen, and in vitro and in vivo transgene expression from immobilized and released lentiviruses were assessed. Cells seeded onto biomaterials loaded with lentiviruses yielded titer‐dependent transgene expression in vitro . Lentivirus activity on both biomaterials was maintained for at least 5 days. When implanted subcutaneously in rats, POC and PGS with immobilized lentivirus exhibited sustained and localized transgene expression for at least 5 weeks. This research demonstrates that lentivirus immobilization on POC and PGS is feasible and potentially useful for a variety of tissue engineering and regenerative medicine applications. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.