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In vitro biological study of gelatin/PLG nanocomposite using MCF‐7 breast cancer cells
Author(s) -
Haroun Ahmed A.,
AboZeid Mona A.,
Youssef Ahmed M.,
GamalEldeen Amira
Publication year - 2013
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34441
Subject(s) - materials science , gelatin , mcf 7 , nanocomposite , scanning electron microscope , thermogravimetric analysis , nuclear chemistry , cancer cell , nanotechnology , chemical engineering , composite material , chemistry , organic chemistry , cancer , medicine , human breast , engineering
This work aimed to evaluate new materials to be carried out as scaffolds using breast cancer cells MCF‐7. These new nanocomposites were prepared through blending of gelatin with poly ( DL ‐lactide‐ co ‐glycolide) (PLG) in presence of titanium nanowires (TiO 2 ) and cartilage powder (CP). The prepared nanomaterials were characterized using Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscope, and transmitting electron microscope. Moreover, the MCF‐7 cells were in vitro tested with apoptosis/necrosis assay, micronucleus test, and DNA fragmentation and MMT assay. TiO 2 nanowires and CP particles have diameters around 28–128 and 17–20 nm, respectively. These were coated with gelatin matrix. Seeding of MCF‐7 cells with the prepared nanomaterials revealed high cell attachment to their surfaces and they were viable after 72 h. It has been shown that the prepared nanocomposites did not induce necrotic effects on MCF‐7 cells; however, they induced a significant DNA fragmentation in comparison with the nontreated control cells. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.