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Therapeutic effect of zinc‐containing calcium phosphate suspension injection in thermal burn‐rats
Author(s) -
Otsuka Makoto,
Shikamura Masayuki,
Otsuka Kuniko,
Sogo Yu,
Ito Atsuo
Publication year - 2013
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34429
Subject(s) - zinc , calcium , saline , materials science , phosphate , nuclear chemistry , sodium , in vitro , zinc phosphate , chemistry , metallurgy , biochemistry , medicine
Abstract The aim of this study was to evaluate the efficacy of suspensions of zinc‐containing tricalcium phosphate (TCP) in the healing of thermal burns in rats. β‐ZnTCP containing 10 mol % zinc, α‐ZnTCP containing 0.9 mol % zinc, and ZnSO 4 ·(H 2 O) 7 (ZnSO 4 ) were used. The injections were prepared to suspend ZnSO 4 , α‐ZnTCP, and β‐ZnTCP powders in 2 mL of 1% sodium alginate saline solution containing 2 mg of Zn. In vitro Zn release rates were measured in simulated body fluid. The release of Zn from ZnSO 4 was very fast, but that from α‐ZnTCP and β‐ZnTCP was slowed by transformation to hydroxyapatite. The suspensions were injected into group C (control), D1 (ZnSO 4 ), D2 (α‐ZnTCP), and D3 (β‐ZnTCP) rats after thermal burns treatment for 3 h. The area under the curve for the plasma Zn for group D1 was the highest, and the order was groups D1 > D2 ≥ D3 ≥ C. The wounded area (Aw) of group D1 had almost the same profile as that of group C, and the Aw at 18 days was about 20%. In contrast, the Aw of group D2 and D3 decreased, and on day 15 was 8% and 37%, respectively. The results indicated that the healing process was shorter in the rats given α‐ZnTCP and β‐ZnTCP than those given ZnSO 4 or the control. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.