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Bone tissue engineering of induced pluripotent stem cells cultured with macrochanneled polymer scaffold
Author(s) -
Jin GuangZhen,
Kim TaeHyun,
Kim JoongHyun,
Won JongEun,
Yoo SoYoung,
Choi SeongJun,
Hyun Jung Keun,
Kim HaeWon
Publication year - 2013
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34425
Subject(s) - tissue engineering , osteopontin , osteocalcin , biomedical engineering , scaffold , materials science , induced pluripotent stem cell , embryoid body , immunostaining , microbiology and biotechnology , flow cytometry , chemistry , biology , immunology , alkaline phosphatase , immunohistochemistry , biochemistry , embryonic stem cell , medicine , gene , enzyme
A reliable source of osteogenic cells is an essential factor for bone tissue engineering. In this study, human‐induced pluripotent stem cells (hiPSCs) without an embryoid body step were cultured in macrochanneled poly(caprolactone) (PCL) scaffolds prepared using a robotic dispensing technique, after which osteogenesis was promoted by the addition of exogenous osteogenic factors. The osteogenesis of the hiPSCs was demonstrated based on the detection of osteogenic molecules, such as osteopontin, using flow cytometry analysis, quantitative polymerase chain reaction and western blotting. Thereafter, the cell‐scaffold constructs were transplanted into the subcutaneous site of male athymic mice. At 4 weeks after implantation, histological assays (hematoxylin & eosin staining, Alizarin red staining, and osteocalcin immunostaining) were conducted to determine the bone induction of hiPSCs. The results indicated a production of pronounced levels of extracellular matrices and their mineral deposition within the cell‐scaffold implant, suggesting possible in vivo bone induction by the hiPSCs‐based tissue engineering approach. The results presented here provide useful information regarding the tissue engineering of bone utilizing hiPSCs in conjunction with cell‐supporting scaffolds. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.