High yield isolation of BMP‐2 from bone and  in vivo  activity of a combination of BMP‐2/TGF‐β 1 | Zendy

Sibiya Sibusiso J. | Zendy; Olivier Eugene I. | Zendy; Duneas Nicolaas | Zendy

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High yield isolation of BMP‐2 from bone and in vivo activity of a combination of BMP‐2/TGF‐β 1

Author(s) -

Sibiya Sibusiso J.,

Olivier Eugene I.,

Duneas Nicolaas

Publication year - 2013

Publication title -

journal of biomedical materials research part a

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.849

H-Index - 150

eISSN - 1552-4965

pISSN - 1549-3296

DOI - 10.1002/jbm.a.34365

Subject(s) - in vivo , bone morphogenetic protein , matrix (chemical analysis) , materials science , bone morphogenetic protein 2 , transforming growth factor , yield (engineering) , biological activity , chromatography , biophysics , in vitro , microbiology and biotechnology , biology , biochemistry , chemistry , metallurgy , gene

A high‐yield purification procedure for protein fractions derived from porcine bone matrix extracts is described, which has a high abundance of bone morphogenetic protein‐2 (BMP‐2). Naturally derived pBMP‐2, ∼5 μg per kilogram of porcine bone matrix, was isolated by using a 300 kDa membrane before chromatographic processing on heparin affinity media. The elution of pBMP‐2 and transforming growth factor‐β 1 (TGF‐β 1 ) revealed morphogen peaks that were unresolved on Prosep ® medium, but resolved on hydroxyapatite medium. Antagonism was observed in animal studies when the two proteins were combined in specific doses. The TGF‐β 1 fraction alone was not active in the rodent heterotopic in vivo bioassay, confirming previously obtained results. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 641–646, 2013.

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