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Study on in vitro release and cell response to alendronate sodium‐loaded ultrahigh molecular weight polyethylene loaded with alendronate sodium wear particles to treat the particles‐induced osteolysis
Author(s) -
Qu Shuxin,
Bai Yinlong,
Liu Xiaomin,
Fu Rong,
Duan Ke,
Weng Jie
Publication year - 2013
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34327
Subject(s) - materials science , osteolysis , particle size , osteoblast , polyethylene , in vitro , particle (ecology) , bone resorption , composite material , chemical engineering , dentistry , medicine , chemistry , biochemistry , oceanography , engineering , geology
The aim of this study is to investigate in vitro release and cell response to wear particles of ultrahigh molecular weight polyethylene loaded with alendronate sodium (UHMWPE‐ALN), a potent bone resorption inhibitor. Wear particles of UHMWPE‐ALN with different ALN contents (0.5 wt % or 1.0 wt %) and size ranges (<45 μm or 45–75 μm) were cocultured with macrophages (RAW264.7) and osteoblasts (MC3T3‐E1), respectively. The in vitro ALN release was divided into three stages: an initial burst release, subsequent rapid release, and final slow release. The particle size and ALN content of UHMWPE‐ALN wear particles affected the in vitro release mainly during initial burst and rapid release. Compared with the control cells, UHMWPE‐ALN wear particles stimulated a significant elevation of tumor necrosis factor‐alpha (TNF‐α) release from macrophages but had no obvious effect on interleukin‐6 release. However, this stimulation of TNF‐α release could be reduced by ALN released from UHMWPE‐ALN wear particles. The wear particle size had stronger effect of on the macrophages compared with the ALN concentration. After coculture with UHMWPE‐ALN wear particles, osteoblast proliferation and alkaline phosphatase activities increased moderately with the increase in particle sizes and ALN concentrations. These results suggest that incorporation of ALN in UHMWPE‐ALN may be an effective approach to prevent or reduce particles‐induced osteolysis. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.