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Construction and hemocompatibility study of highly bioactive heparin‐functionalized surface
Author(s) -
Yang ZhiLu,
Zhou Shuo,
Lu Lei,
Wang Xin,
Wang Jin,
Huang Nan
Publication year - 2012
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34247
Subject(s) - heparin , materials science , partial thromboplastin time , surface modification , adsorption , allylamine , adhesion , platelet adhesion , protonation , chemical engineering , biomedical engineering , nuclear chemistry , chromatography , polymer , platelet , organic chemistry , composite material , chemistry , biochemistry , medicine , ion , polyelectrolyte , engineering , immunology
A simple method is developed to construct anticoagulant surfaces via passive adsorption of heparin onto the protonated plasma‐polymerized allylamine (PPAam) films from phosphate‐buffered saline (PBS). These protonated PPAam surfaces are found to have high affinity to heparin. Importantly, the heparin‐functionalized PPAam (Hep–PPAam) surfaces show good retention of heparin after long‐term immersion in PBS. The Hep–PPAam surface prolongs the activated partial thromboplastin time for about 20 s as compared to 316L stainless steel even though the adsorption amount of heparin is only about 300 ng/cm 2 . This indicates that the heparin bound to the protonated PPAam surfaces in this way maintains a high bioactivity. Blood platelet adhesion and activation on this surface is remarkably reduced and adsorption and activation of fibrinogen is inhibited. Thus, Hep–PPAam surface modification leads to a significant improve of the hemocompatibility. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 100A:3124–3133, 2012.