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Multiscale grooved titanium processed with femtosecond laser influences mesenchymal stem cell morphology, adhesion, and matrix organization
Author(s) -
Dumas Virginie,
Rattner Aline,
Vico Laurence,
Audouard Eric,
Dumas Jean Claude,
Naisson Pierre,
Bertrand Philippe
Publication year - 2012
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34239
Subject(s) - materials science , nanostructure , femtosecond , focal adhesion , mesenchymal stem cell , nanotechnology , titanium , laser , adhesion , nanoscopic scale , cell adhesion , biomedical engineering , optics , composite material , cell , medicine , physics , genetics , biology , microbiology and biotechnology , metallurgy
The femtosecond laser processing enabled the structuring of six types of surfaces on titanium‐6aluminium‐4vanadium (Ti‐6Al‐4V) plates. The obtained hierarchical features consisted of a combination of microgrooves and oriented nanostructures. By adjusting beam properties such as laser polarization, the width of the microgrooves (20 or 60 μm) and the orientation of the nanostructures (parallel or perpendicular to the microgrooves) can be precisely controlled. Mesenchymal stem cells (MSCs) grown on these structured surfaces produced cytoplasmic extensions with focal contacts, while on the smooth titanium, the cells were found to be well spread and without any focal contact 12 h postseeding. The 600‐nm wide nanostructures on their own were sufficient to orient the MSCs. For the multiscale structured areas, when the orientation of the nanostructures was orthogonal in relation to the microgrooves, there was an important decrease in or even a loss of cell alignment signifying that cells were sensitive to the directional nanostructures in the microgrooves. At 7 days, cell proliferation was not affected but the direction of nanostructures controlled the matrix organization. The ultrafast laser, as a new method for producing micro‐nanohybrid surfaces, is a promising approach to promote desired tissue organization for tissue engineering. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 100A:3108–3116, 2012.