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Interplay between protein‐modified surface and functional response of osteoblasts
Author(s) -
Chaudhary P.,
Pesacreta T. C.,
Misra R. D. K.
Publication year - 2012
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34232
Subject(s) - vinculin , fibronectin , protein adsorption , materials science , biophysics , microbiology and biotechnology , adsorption , osteoblast , cell , substrate (aquarium) , fibronectins , actin , nanotechnology , cell adhesion , biochemistry , adhesion , biology , chemistry , in vitro , ecology , organic chemistry , composite material , polymer
The objective of the study is to elucidate the interplay between fibronectin‐metal hybrid surfaces and osteoblast function. The practical relevance is that a significant initial step in the process of prosthetic integration within a physiological system is the rapid adsorption of proteins, including fibronectin, on the surface of biomedical device. Here, we compare and contrast the cell–substrate interactions on bare and protein‐modified surfaces. The protein adsorption on the surface was beneficial in favorably modulating biological functions including cell attachment, proliferation, and viability. Additionally, immunofluorescence studies demonstrated stronger vinculin signals associated with actin stress fibers in the outer regions of the cells and cellular extensions on protein‐adsorbed surfaces. These results support the hypothesis that protein adsorption on artificial biomedical devices can promote bioactivity and regulate biological functions. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 100A:3157–3166, 2012.