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Titanium surfaces with nanotopography modulate cytokine production in cultured human gingival fibroblasts
Author(s) -
SchwartzFilho Humberto Osvaldo,
Morandini Ana Carolina Faria,
RamosJunior Erivan Schnaider,
Jimbo Ryo,
Santos Carlos Ferreira,
Marcantonio Elcio,
Wennerberg Ann,
Marcantonio Rosemary Adriana Chiérici
Publication year - 2012
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34200
Subject(s) - nanotopography , materials science , transforming growth factor , cytokine , titanium , acid etching , etching (microfabrication) , nanotechnology , microbiology and biotechnology , immunology , biology , metallurgy , composite material , layer (electronics)
Abstract Implant topography is an important factor that influences many cell types. To understand the role of topography in the inflammatory events, we evaluated the response of human gingival fibroblasts (HGFs) by the release pattern of cytokines. HGFs were cultured on Ti discs for 24 and 48 h. Four different surface treatments were used: machining method (turned), blasting followed by an acid‐etching method (BAE), oxidative nanopatterning (ON) method, and an association of blasting followed by an acid‐etching plus oxidative nanopatterning (BAE+ON) method. Extracellular levels of IL‐6, IL‐8, transforming growth factor beta (TGF‐β), IL‐4, and IL‐10 were measured by enzyme‐linked immunosorbant assay. Increased levels of IL‐6 and IL‐8 were observed in all surfaces after 24 h which decreased after 48 h. BAE, ON, and BAE+ON surfaces showed a reduction in IL‐6 levels compared with the turned after 48 h ( p < 0.05). On one hand, IL‐8 production was lower in BAE+ON in comparison to the turned surface ( p < 0.05). On the other hand, IL‐4 showed increased levels with 48 h, which were significantly different between turned, BAE, and ON surfaces, but not with BAE+ON. Additionally, TGF‐β and IL‐10 production were not detected. This study indicates that nanotopography might be important in the modulation of the inflammatory response in cultured HGFs. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A 100A:2629–2636, 2012.