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Synthesis of cationic magnetic nanoparticles and evaluation of their gene delivery efficacy in Hep G2 cells
Author(s) -
Chen JuiTse,
Ahmed Marya,
Liu Qingxia,
Narain Ravin
Publication year - 2012
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34176
Subject(s) - materials science , cationic polymerization , nanoparticle , gene delivery , magnetic nanoparticles , hep g2 , nanotechnology , genetic enhancement , cell culture , gene , biochemistry , biology , polymer chemistry , genetics
Well‐defined cationic polymers were synthesized via the reversible addition fragmentation chain transfer polymerization and were subsequently used as stabilizer for the synthesis of cationic iron‐oxide magnetic nanoparticles via the coprecipitation method. The surface‐coated iron‐oxide nanoparticles made stable suspension in water and were characterized by a range of techniques such as dynamic light scattering, transmission electron microscopy, and thermogravimetric analysis. The presence of protonated amine and carbohydrate residues onto the surface of the nanoparticles allowed their facile complexation with DNA and the resulting nanocomplexes were then studied for their efficacy as DNA carriers in Hep G2 cells. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 100A: 2342–2347, 2012.