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Stirred flow bioreactor modulates chondrocyte growth and extracellular matrix biosynthesis in chitosan scaffolds
Author(s) -
García Cruz Dunia Mercedes,
SalmerónSánchez Manuel,
GómezRibelles José Luis
Publication year - 2012
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34174
Subject(s) - chondrogenesis , extracellular matrix , chondrocyte , aggrecan , scaffold , materials science , bioreactor , type ii collagen , biophysics , tissue engineering , matrix (chemical analysis) , cartilage , biomedical engineering , cell culture , microbiology and biotechnology , chemistry , anatomy , biology , composite material , osteoarthritis , articular cartilage , pathology , medicine , alternative medicine , organic chemistry , genetics
The aim of this study is to show the favorable effect of simple dynamic culture conditions on chondrogenesis of previously expanded human chondrocytes seeded in a macroporous scaffold with week cell‐pore walls adhesion. We obtained enhanced chondrogenesis by the combination of chitosan porous supports with a double micro‐ and macro‐pore structure and cell culture in a stirring bioreactor. Cell‐scaffold constructs were cultured under static or mechanically stimulated conditions using an intermittent stirred flow bioreactor during 28 days. In static culture, the chondrocytes were homogeneously distributed throughout the scaffold pores; cells adhered to the scaffold pore walls, showed extended morphology and were able to proliferate. Immunofluorescense and biochemical assays showed abundant type I collagen deposition at day 28. However, the behavior of chondrocytes submitted to mechanical stimuli in the bioreactor was completely different. Mechanical loading influenced cell morphology and extracellular matrix composition. Under dynamic conditions, chondrocytes kept their characteristic phenotype and tended to form cell aggregates surrounded by a layer of the main components of the hyaline cartilage extracellular matrix, type II collagen, and aggrecan. An enhanced aggrecan and collagen type II production was observed in engineered cartilage constructs cultured under stirred flow compared with those cultured under static conditions. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 100A: 2330–2341, 2012.