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Stabilization of collagen tissues by photocrosslinking [Note a. a p < 0.01. ...]
Author(s) -
Vashi Aditya V.,
Werkmeister Jerome A.,
Vuocolo Tony,
Elvin Christopher M.,
Ramshaw John A. M.
Publication year - 2012
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34164
Subject(s) - materials science , periodate , sodium , persulfate , potassium persulfate , shrinkage , membrane , biophysics , polymerization , catalysis , biochemistry , chemistry , composite material , biology , polymer , metallurgy
Photocrosslinking, using 2 m M Ru II (bpy) 3 Cl 2 and various concentrations of sodium persulfate with irradiation by blue light, ∼455 nm, has been shown to be a rapid and effective method for crosslinking various tissues: tendon, amnion membrane, pericardium, and heart valve leaflet. The presence of new crosslinking was demonstrated by the increase in the shrinkage temperature of these tissues. In all the cases, increase in the shrinkage temperatures were seen, although at higher sodium persulfate concentrations, for example, 100 m M , both with and without the Ru II (bpy) 3 Cl 2 catalyst, some degradation of the collagenous tissues was found. The effectiveness of this photocrosslinking method when used with tissues was also shown through the increase in the break strength of tissues after crosslinking, and by the reduction of protein that could be extracted by urea. In solution studies, dityrosine has been shown to be formed during photocrosslinking. With tissues, Western blotting showed the presence of new dityrosine crosslinked proteins. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 100A: 2239–2243, 2012.