Premium
Immobilization of the direct thrombin inhibitor‐bivalirudin on 316L stainless steel via polydopamine and the resulting effects on hemocompatibility in vitro
Author(s) -
Lu Lei,
Li QuanLi,
Maitz Manfred F.,
Chen JiaLong,
Huang Nan
Publication year - 2012
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34143
Subject(s) - materials science , quartz crystal microbalance , partial thromboplastin time , surface modification , adsorption , thrombin time , thrombin , platelet activation , bivalirudin , x ray photoelectron spectroscopy , nuclear chemistry , coating , hirudin , prothrombin time , fibrinogen , platelet , biomedical engineering , chemical engineering , nanotechnology , chemistry , organic chemistry , biochemistry , surgery , medicine , immunology , psychiatry , percutaneous coronary intervention , myocardial infarction , engineering
Abstract Bivalirudin (BV), a peptidic direct thrombin inhibitor, derived from hirudin, has gained increasing interest in clinical anticoagulant therapy in the recent years. In this work, a hemocompatible surface was prepared by immobilization of BV on 316L stainless steel (SS) using a bonding layer of polydopamine (DA). X‐ray photoelectron spectroscopy (XPS) was used to determine the chemical composition of the surfaces to characterize polydopamine intermediate layer and the immobilized BV. The quantity of bound BV was measured by quartz crystal microbalance (QCM). The hemocompatibility in vitro was evaluated by coagulating time of activated partial thromboplastin time (aPTT) and prothrombin time (PT) assay, platelet adhesion and activation, fibrinogen adsorption, and activation and whole blood test. The effect of sterilizing method on the bioactivity of immobilized BV was also evaluated. The results showed that BVs were successfully immobilized on SS surface with the DA interlayer at a density of 98 ng/cm 2 . BV coating surface prolonged aPTT and PT, inhibited the activation of platelet and fibrinogen significantly. Sterilization by ultraviolet radiation was possible with only marginal loss of activity. Thus, the approach described here may provide a basis for the preparation of 316L SS surface modification for use in cardiovascular implants. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 100A: 2421–2430, 2012.