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A novel method to fabricate thermoresponsive microstructures with improved cell attachment/detachment properties
Author(s) -
He XiaoLing,
Nie PingPing,
Chen BiZhou,
Li XiangXu,
Chen Li,
Guo Gang,
Zhang Rui
Publication year - 2012
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34138
Subject(s) - materials science , lower critical solution temperature , thermoresponsive polymers in chromatography , fourier transform infrared spectroscopy , x ray photoelectron spectroscopy , adhesion , chemical engineering , microstructure , polystyrene , polymerization , substrate (aquarium) , grafting , cell adhesion , polymer chemistry , polymer , composite material , phase (matter) , chemistry , copolymer , organic chemistry , oceanography , reversed phase chromatography , geology , engineering
A novel, simple, and rapid method to fabricate thermoresponsive micropatterned substrate for cell adhesion, growth, and thermally induced detachment was developed. Thermoresponsive polymer, poly( N ‐isopropylacrylamide) (PNIPAAm), was grafted onto the surface of polystyrene (PS) film with microstructure by plasma‐induced graft polymerization technique. The thermoresponsive micropatterned films were characterized by attenuated total reflectance‐Fourier transform infrared (ATR‐FTIR) spectroscopy, hydrogen nuclear magnetic resonance ( 1 H NMR) spectroscopy, X‐ray photoelectron spectroscopy (XPS) and scanning electron microscope (SEM). These results indicated that the grafting ratio of PNIPAAm increased with increasing roughness of PS film. However, the microstructures on the substrate were not affected by grafted PNIPAAm. The optimal grafting conditions, such as plasma treatment time, monomer concentration, graft polymerization time, and graft medium were investigated. The thermoresponsive micropatterned films were investigated with the fibroblast cell (L929) adhesion, proliferation, and thermally induced detachment assay. The microstructure on the thermoresponsive micropatterned substrate facilitated cell adhesion above the lower critical solution temperature (LCST) of PNIPAAm and cell detachment below the LCST. Moreover, it can be used to regulate cell organization and tissue growth. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A 2012.