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Does the tissue engineering architecture of poly(3‐hydroxybutyrate) scaffold affects cell–material interactions?
Author(s) -
Masaeli Elahe,
Morshed Mohammad,
Rasekhian Parsa,
Karbasi Saeed,
Karbalaie Khadije,
Karamali Fereshte,
Abedi Daryoush,
Razavi Shahnaz,
JafarianDehkordi Abbas,
NasrEsfahani Mohammad Hossein,
Baharvand Hossein
Publication year - 2012
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34131
Subject(s) - materials science , scaffold , biocompatibility , tissue engineering , ultimate tensile strength , electrospinning , biomedical engineering , contact angle , extracellular matrix , mesenchymal stem cell , composite material , polymer , chemistry , microbiology and biotechnology , medicine , biochemistry , metallurgy , biology
A critical element in tissue engineering involves the fabrication of a three‐dimensional scaffold. The scaffold provides a space for new tissue formation, supports cellular ingrowth, and proliferation and mimics many roles of the extracellular matrix. Poly(3‐hydroxybutyrate) (PHB) is the most thoroughly investigated member of the polyhydroxyalkanoates (PHAs) family that has various degrees of biocompatibility and biodegradability for tissue engineering applications. In this study, we fabricated PHB scaffolds by utilizing electrospinning and salt‐leaching procedures. The behavior of monkey epithelial kidney cells (Vero) and mouse mesenchymal stem cells (mMSCs) on these scaffolds was compared by the MTS assay and scanning electron microscopy. Additionally, this study investigated the mechanical and physical properties of these scaffolds by measuring tensile strength and modulus, dynamic contact angle and porosity. According to our results, the salt‐leached scaffolds showed more wettability and permeability, but inferior mechanical properties when compared with nanofibrous scaffolds. In terms of cell response, salt‐leached scaffolds showed enhanced Vero cell proliferation, whereas both scaffolds responded similarly in the case of mMSCs proliferation. In brief, nanofibrous scaffolds can be a better substrate for cell attachment and morphology. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A 2012.