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Adipogenesis on biphasic calcium phosphate using rat adipose‐derived mesenchymal stem cells: In vitro and in vivo
Author(s) -
Venugopal Balu,
Fernandez Francis B.,
Babu Suresh S.,
Harikrishnan V. S.,
Varma Harikrishna,
John Annie
Publication year - 2012
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34082
Subject(s) - adipose tissue , adipogenesis , mesenchymal stem cell , in vivo , tissue engineering , biomedical engineering , transplantation , scaffold , materials science , stem cell , microbiology and biotechnology , biology , surgery , medicine , endocrinology
Developing adipose tissue‐engineered construct to mend soft tissue defects arising from traumatic injury, tumor resections, and maxillofacial abnormalities is of prime importance in plastic and reconstructive surgical procedures. It is apparent that the clinical outcome of classic techniques like adipose tissue transplantation is unpredictable, with graft resorption, lack of vascularization, and impaired functionality. In this prospective, the concept of tissue engineering was adopted to fabricate a combination product with biphasic calcium phosphate (BCP) and rat adipose‐derived mesenchymal stem cells (ASCs) toward the development of an adipose tissue construct. BCP, a combination of hydroxyapatite and α‐tricalcium phosphate, was characterized for its physiochemical properties, and ASCs were characterized for their stemness. The cell–ceramic interactions were demonstrated in vitro , whereas adipogenesis was picturesquely depicted by Nile red‐stained multilocular adipocyte‐like cells. Subsequently, the three‐dimensional cell–ceramic‐engineered construct was implanted in the rat dorsal muscle for a period of 3 weeks to demonstrate the efficacy of the tissue construct in vivo . Interestingly, the histology of the postimplanted tissue construct revealed the distribution of chicken wire net‐like fat cells within the vicinity of the construct. The efficacy of cell transplantation via the scaffold was traced using fluorescent in situ hybridization by labeling the Y chromosome. Thus, the ceramic‐based construct may be a good option for reconstruction therapies. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2012.