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Preparation of a biomimetic nanocomposite scaffold for bone tissue engineering via mineralization of gelatin hydrogel and study of mineral transformation in simulated body fluid
Author(s) -
Azami Mahmoud,
Moosavifar Mir Javad,
Baheiraei Nafiseh,
Moztarzadeh Fathollah,
Ai Jafar
Publication year - 2012
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34074
Subject(s) - gelatin , materials science , amorphous calcium phosphate , brushite , nanocomposite , simulated body fluid , biomineralization , biocompatibility , scaffold , nanocrystalline material , chemical engineering , bone tissue , porosity , calcium , tissue engineering , self healing hydrogels , biomedical engineering , composite material , nanotechnology , polymer chemistry , chemistry , organic chemistry , metallurgy , scanning electron microscope , medicine , engineering
In this study, double diffusion method in a physiologically relevant environment was used to prepare a biomimetic gelatin‐amorphous calcium phosphate nanocomposite scaffold. The precipitated calcium phosphate within gelatin as well as produced nanocomposite scaffolds were characterized by the commonly used bulk techniques. The results showed that nanocomposite scaffolds were porous with three‐dimensionally interconnected microstructure, pore size ranging from 150 to 350 μm. Porosity was about 82% and nanocrystalline precipitated minerals were dispersed evenly among gelatin fibers. A mineral containing amorphous calcium phosphate and brushite precipitate was formed within the gelatin matrix at 4°C. After incubation in SBF solution at 37°C for 5 days, the mineral phase was transformed to nanocrystalline hydroxyapatite. It should be noted that precursor phases inside a scaffold implanted into the body can result in biomimetic conversion of precursors to hydroxyapatite that is very similar to the bone mineral and has a profound level of biocompatibility. Thus, our results highlight the potential use of engineered biomimetic bone tissue scaffolds in the bone tissue repair process. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 2012.