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Novel biodegradable three‐dimensional macroporous scaffold using aligned electrospun nanofibrous yarns for bone tissue engineering
Author(s) -
Cai YouZhi,
Zhang GuoRong,
Wang LinLin,
Jiang YangZi,
Ouyang HongWei,
Zou XiaoHui
Publication year - 2012
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34063
Subject(s) - scaffold , materials science , biomedical engineering , polycaprolactone , mesenchymal stem cell , tissue engineering , nanofiber , electrospinning , bone tissue , nanotechnology , composite material , microbiology and biotechnology , medicine , biology , polymer
This study aimed to develop a practical three‐dimensional (3D) macroporous scaffold from aligned electrospun nanofibrous yarns for bone tissue engineering. A novel 3D unwoven macroporous nanofibrous (MNF) scaffold was manufactured with electrospun poly( L ‐lactic acid) and polycaprolactone (w/w 9:1) nanofibers through sequential yarns manufacture and honeycombing process at 65°C. The efficacy of 3D MNF scaffold for bone formation were evaluated using human embryonic stem cell‐derived mesenchymal stem cells (hESC‐MSCs) differentiation model and rabbit tibia bone defect model. In vitro , more cell proliferation and cell ingrowth were observed in 3D MNF scaffold. Moreover, calcium deposit was obviously detected in vitro differentiation of hESC‐MSCs. In vivo , histology and X‐ray showed that 3D MNF scaffold treated bone defect had fine 3D bony tissue formation around the scaffold as well as inside the scaffold at 3 weeks and 6 weeks. This study demonstrated that 3D MNF scaffold provides a structural support for hESC‐MSCs growth and guides bone formation suggesting that this novel strategy successfully makes use of electrospun fibers for bone tissue engineering, which may help realize the clinical translation of electrospun nanofibers for regenerative medicine in future. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2012.