Premium
Endothelial cell adhesion and proliferation to PEGylated polymers with covalently linked RGD peptides
Author(s) -
Wang Xin,
Heath Daniel E.,
Cooper Stuart L.
Publication year - 2012
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34026
Subject(s) - materials science , peptide , methacrylate , polymer chemistry , ethylene glycol , peg ratio , polymer , adhesion , human umbilical vein endothelial cell , comonomer , cell adhesion , protein adsorption , biophysics , umbilical vein , biochemistry , copolymer , organic chemistry , chemistry , in vitro , finance , economics , composite material , biology
A nonfouling peptide grafted polymer was synthesized that can promote endothelial cell (EC) binding. The polymer was composed of hexyl methacrylate, methyl methacrylate, poly(ethylene glycol) methacrylate, and CGRGDS peptide. The peptide was incorporated into the polymer system either by a chain transfer reaction or by coupling to an acrylate‐PEG‐ N ‐hydroxysuccinimide (NHS) comonomer. The introduction of PEG chains minimizes protein adsorption. Human umbilical vein ECs and endothelial colony forming cells were cultured on these surfaces in short term and long‐term studies. A difference in number and morphology of ECs was observed depending on the method of peptide incorporation. Both cell types adhered better to polymer films containing NHS coupled RGD peptide after 2 h even in the presence of albumin but significant cell detachment occurred after 4 days. Polymer solutions were electrospun into fibrous scaffolds. Both nonfouling and peptide binding characteristics were retained after processing. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.