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Confocal Raman microspectroscopic study of folate receptor‐targeted delivery of 6‐mercaptopurine‐embedded gold nanoparticles in a single cell
Author(s) -
Park Jin,
Jeon Won Il,
Lee So Yeong,
Ock KwangSu,
Seo Ji Hye,
Park Jinho,
Ganbold ErdeneOchir,
Cho Keunchang,
Song Nam Woong,
Joo SangWoo
Publication year - 2012
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.33294
Subject(s) - folate receptor , materials science , colloidal gold , conjugated system , drug delivery , hela , biophysics , cancer cell , confocal microscopy , viability assay , photothermal therapy , nanotechnology , nanoparticle , cell , biochemistry , chemistry , medicine , cancer , biology , microbiology and biotechnology , composite material , polymer
Abstract We investigate the cellular uptake behaviors and efficacy of folate‐coated gold nanoparticles (AuNPs) for the targeted drug delivery system in human cancer cells. Folate‐conjugated AuNPs embedded with a purine analogue cancer drug of 6‐mercaptopurine (6MP) were assembled via a 1‐ethyl‐3‐[3‐dimethylaminopropyl] carbodiimide hydrochloride (EDC) coupling reaction between the amino group of 4‐aminobenzenethiol (ABT) and the carboxyl group of folic acid. The assembly of folate and 6MP on AuNPs has been examined by absorption spectroscopy, transmission electron microscopy (TEM), and confocal Raman spectroscopy. The internalization of the conjugated AuNPs inside the folate receptor‐positive HeLa and KB cells was checked by TEM and dark‐field microscopy (DFM) combined with label‐free confocal spectroscopy over the depth variable z at a micrometer resolution. DFM live cell imaging of folate‐conjugated AuNPs in HeLa cells indicated that the targeted AuNPs appeared to attach on the cell surfaces and enter into the cell with an hour. The cell viability was also compared to estimate the efficacy of folate‐conjugated AuNP delivery systems. Folate receptor‐targeted AuNP systems appeared to decrease cancer cell viability both in vitro and in vivo more than did the use of the 6MP‐coated AuNPs drug without any targeting systems. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2012.