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Fabrication of protein micropatterns using a functional substrate with convertible protein‐adsorption surface properties
Author(s) -
Yamazoe Hironori
Publication year - 2012
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.33279
Subject(s) - materials science , protein adsorption , adsorption , quartz crystal microbalance , albumin , chemical engineering , substrate (aquarium) , biochip , surface modification , coating , fabrication , polymer , nanotechnology , composite material , chemistry , organic chemistry , biochemistry , medicine , oceanography , alternative medicine , pathology , engineering , geology
A functional substrate capable of regulating protein adsorption was prepared using a crosslinked albumin (cl‐albumin) film for use in the fabrication of protein micropatterns. The adsorption of proteins with different characteristics onto cl‐albumin film, including serum proteins, serum albumin, and lysozyme, was investigated using a quartz crystal microbalance. The results showed that surfaces coated with cl‐albumin film are highly resistant to protein adsorption, regardless of protein charge and rigidity. In addition, this adsorption‐resistance property can be easily converted to promote protein adsorption by exposing the cl‐albumin film to a charged polymer solution. By combining the convertible surface property of cl‐albumin film and inkjet printing techniques, a precise protein micropattern was successfully fabricated on the substrate. Protein adsorption onto the wall surface of microchannels could also be suppressed or promoted by coating the surface with cl‐albumin film. This approach will aid in the development of biomaterials carrying protein micropatterns, such as biosensors, biochips, and cellular scaffolds. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.