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Magnetic field‐induced acceleration of the accumulation of magnetic iron oxide nanoparticles by cultured brain astrocytes
Author(s) -
Lamkowsky MarieChristin,
Geppert Mark,
Schmidt Maike M.,
Dringen Ralf
Publication year - 2012
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.33263
Subject(s) - magnetic field , materials science , iron oxide nanoparticles , incubation , brain cell , iron oxide , magnetic nanoparticles , magnet , biophysics , internalization , nanoparticle , nuclear magnetic resonance , nanotechnology , cell , biochemistry , biology , microbiology and biotechnology , metallurgy , physics , quantum mechanics
Magnetic iron oxide nanoparticles (Fe‐NPs) are considered for various biomedical and neurobiological applications that involve the presence of external magnetic fields. However, little is known on the effects of a magnetic field on the uptake of such particles by brain cells. Cultured brain astrocytes accumulated dimercaptosuccinate‐coated Fe‐NP in a time‐, temperature‐, and concentration‐dependent manner. This accumulation was strongly enhanced by the presence of the magnetic field generated by a permanent neodymium iron boron magnet that had been positioned below the cells. The magnetic field‐induced acceleration of the accumulation of Fe‐NP increased almost proportional to the strength of the magnetic field applied, increasing the cellular‐specific iron content from an initial 10 nmol/mg protein within 4 h of incubation at 37°C to up to 12,000 nmol/mg protein. However, presence of a magnetic field also increased the amounts of iron that attached to the cells during incubation with Fe‐NP at 4°C. These results suggest that the presence of an external magnetic field promotes in cultured astrocytes both the binding of Fe‐NP to the cell membrane and the internalization of Fe‐NP. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.