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Synthesis and characterization of injectable, thermosensitive, and biocompatible acellular bone matrix/poly(ethylene glycol)‐poly (ε‐caprolactone)‐poly(ethylene glycol) hydrogel composite
Author(s) -
Ni PeiYan,
Fan Min,
Qian ZhiYong,
Luo JingCong,
Gong ChangYang,
Fu ShaoZhi,
Shi Shuai,
Luo Feng,
Yang ZhiMing
Publication year - 2012
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.33262
Subject(s) - biocompatibility , materials science , ethylene glycol , copolymer , composite number , tissue engineering , caprolactone , polymer chemistry , biomedical engineering , chemical engineering , polymer , composite material , medicine , engineering , metallurgy
In orthopedic tissue engineering, the extensively applied acellular bone matrix (ABM) can seldom be prefabricated just right to mold the cavity of the diverse defects, might induce severe inflammation on account of the migration of small granules and usually bring the patients great pain in the treatment. In this study, a new injectable thermosensitive ABM/PECE composite with good biocompatibility was designed and prepared by adding the ABM granules into the triblock copolymer poly(ethylene eglycol)‐poly(ε‐caprolactone)‐poly(ethylene eglycol) (PEG‐PCL‐PEG, PECE). The PECE was synthesized by ring‐opening copolymerization and characterized by 1 H NMR. The ABM was prepared by acellular treatment of natural bone and ground to fine granules. The obtained ABM/PECE composite showed the most important absorption bands of ABM and PECE copolymer in FT‐IR spectroscopy and underwent sol–gel phage transition from solution to nonflowing hydrogel at 37°C. SEM results indicated that the ABM/PECE composite with different ABM contents all presented similar porous 3D structure. ABM/PECE composite presented mild cytotoxicity to rat MSCs in vitro and good biocompatibility in the BALB/c mice subcutis up to 4 weeks. In conclusion, all the results confirmed that the injectable thermosensitive ABM/PECE composite was a promising candidate for orthopedic tissue engineering in a minimally‐invasive way. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.